Figure 5
Expression of the BACE genes is controlled by the clock.
(A) Schematic plot of the promoters of the BACE genes, including four possible E-boxes located in the 1.4-kb BACE2 promoter and one D-box located in the 1-kb BACE1 promoter. (B) Left pane: transfection of E4BP4 inhibited the transcription of Bace1 in a dose-dependent manner. Right pane: The CLOCK:BMAL1 complex activated the expression of Bace2 in a dose-dependent manner. (C) The expression of Per2 and Bace2 between Clock mutant and WT mice in hippocampus. Clockdelta19/delta19 and WT mice (n = 3) were sacrificed at ZT5 and ZT7 under the LD condition; the expressions of Per2 and Bace2 were decreased at ZT5 and ZT17 in the Clockdelta19/delta19 mice due to the deficiency of the Clock gene; Bar graph (mean ± s.e.m.) of relative gene expression (n = 3, N.S. P > 0.05; *P < 0.05; **P < 0.01, student’s t-test).
