Figure 6
The rhythmicity of Bace2 and ApoE expression is altered in the hippocampus of APP/PS1dE9 mice.
(A) Thioflavin T staining of WT and APP/PS1dE9 mice. Fibrillary amyloid plaques existed only in the brains of the APP/PS1dE9 mice (Red asterisks indicate fibrillary amyloid plaques). (B) The diurnal expression pattern of the orexin precursor gene is altered in the hypothalamus of APP/PS1dE9 mice. Expression of orexin precursor mRNA was analyzed by qPCR in the hypothalamus of WT and APP/PS1dE9 mice. The expression of this gene was higher at ZT5 in APP/PS1dE9 mice compared with aging WT mice; we did not detect any differences in mRNA levels at other time points. (C) The rhythmicity of Bace2 and ApoE expression is altered in the hippocampus of APP/PS1dE9 mice. Left panel: expression of Bace2 shows that this AD-related gene increased dramatically in the hippocampus of APP/PS1dE9 mice. Right panel: ApoE gene expression elevated the expression at ZT5, ZT11, and ZT17. The expression of this gene, which is highly correlated with the metabolism of Aβ, increased slightly in the hippocampus of APP/PS1dE9 mice. (D) Canonical E-box genes such as Dbp, Per1, Per2, and Cry1 changed the rhythmicity in the hippocampus of APP/PS1dE9 mice (n = 3–5, N.S. P > 0.05; *P < 0.05; **P < 0.01, student’s t-test). Top left panel: Dbp; Top right panel: Per1; bottom left panel: Per2; bottom right panel: Cry1.
