Figure 3 | Scientific Reports

Figure 3

From: Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Figure 3

Inhibited adipogenesis in 3T3-L1 cells by LGG EPS without proinflammation induction.

The cell line of 3T3-L1 was treated by isolated EPS from LGG during the Day 0–6 and the sampling was done on Day 6 for TAG and protein assay or total RNA extraction. (A) The profile of lipid droplet formation with Oil red O staining in 3T3-L1 adipocytes. (B) TAG accumulation in 3T3-L1 adipocyte by TAG assay. (C–E) The expression of M1 proinflammatory genes (C) M2 anti-inflammatory genes (D) and lipid metabolism related genes in 3T3-LI cells by q-PCR (E). Data were expressed as mean ± SEM of three independent experiments (n = 3). Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P < 0.05 (*) and P < 0.01 (**).

Back to article page