Figure 4
LGG EPS, not degraded EPS, inhibited the preadipocyte differentiation.
(A) TAG accumulation in 3T3-L1 adipocyte treated by LGG EPS, autoclaved EPS or its degraded products by sulfuric acid. These different forms of EPS or its degraded products were administrated to 3T3-L1 cells during Day 0–6 and the sampling was done on Day 6 for TAG and protein assay. (B) TAG accumulation in 3T3-L1 adipocytes treated by LGG EPS at different differentiation periods as indicated. 3T3-L1 cells were treated by LGG EPS during Day 0–2, Day 3–6 and Day 0–6 and the sampling was done on Day 6 for TAG and protein assay. (C,D) The expression of lipid metabolism related genes when 3T3-L1 cells were treated by LGG EPS during Day 0–1 (namely 24 hours, C) and Day 0–2 (namely 48 hours, D). The sampling was done immediately after the treatments finished for RNA extraction and consequent qPCR. Data were expressed as mean ± SEM of three independent experiments (n = 3). Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P < 0.05 (*) and P < 0.01 (**).
