Figure 3 | Scientific Reports

Figure 3

From: Identification of a two-component Class IIb bacteriocin in Streptococcus pyogenes by recombinase-based in vivo expression technology

Figure 3

Analysis of spbM gene expression in vitro and in vivo.

S. pyogenes MGAS8232 was grown (A) in THY broth to OD600 ~0.2 or ~0.9 (n = 3; early and late in vitro conditions, respectively) or (B) nasally inoculated into mice and harvested at 48 h (n = 4; in vivo conditions) as described in Methods. The black bar represents the geometric mean. (C) Expression of spbM was determined by qRT-PCR and normalized to the gyrA housekeeping gene. The graph shows the mean ± SEM and statistical significance is displayed as *p < 0.05 by ANOVA with Tukey’s Multiple Comparison Test.

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