Figure 4 | Scientific Reports

Figure 4

From: Identification of a two-component Class IIb bacteriocin in Streptococcus pyogenes by recombinase-based in vivo expression technology

Figure 4

The sil-spb loci in S. pyogenes MGAS8232.

(A) Open reading frame map and DNA sequence of the sil and spb operons. The silD gene in MGAS8232 contains a premature stop codon and may potentially encode two separate ORFs labeled silDN and silDC (the latter denoted with hashed lines). The nucleotide and translation products for the spb locus are given below for the indicated region. The location of the DNA region contained within clone IVI156 is indicated. Promoter regions are drawn as black arrows and direct repeats (DR1 and DR2) are annotated as previously determined in S. pyogenes JS9542. Nucleotide numbers are according to the genome sequence of S. pyogenes MGAS8232 (NC_003485.1). (B) Alignment of the Spb peptides from S. pyogenes MGAS8232 and Blp peptides from S. pneumoniae 6A21. Identical (*) and similar (+) residues are indicated. The arrow (↓) indicates the predicted cleavage site of the double-glycine-type leader peptides.

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