Figure 3 | Scientific Reports

Figure 3

From: Identification of G8969>A in mitochondrial ATP6 gene that severely compromises ATP synthase function in a patient with IgA nephropathy

Figure 3

Properties of trans-mitochondrial cell lines carrying various proportion of the G8969>A mutation.

(A) Percentages of the G8969>A mutation in cybrid cell lines. (B) Mitochondrial ultrastructure (magnification ×50,000). In C-98, arrows point to aberrant, torus and horseshoe-shape, mitochondria. (C) Cellular respiration. The OCR (oxygen consumption rates) were evaluated in cybrids with different levels of G8969>A mutation. OSR (oligomycin-sensitive respiration), BR (basal respiration) and MR (maximal respiration). *Stands for comparison to WT, # is comparison to C-19. (D) Levels of reactive oxygen species (ROS). The levels of ROS were assessed using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA)47. Immunofluorescence was visualized by confocal microscopy (left panel) and quantified by flow cytometry and expressed as decimal logarithm versus cell number (right panel). WT, control cells; C-19, C-67, and C-98 are cybrid cell lines containing 19%, 67% and 98% of the G8969>A mutation, respectively. ρ0, cells lacking mtDNA.

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