Table 1 Strains and plasmids used in this study.
Strains | Relevant characteristics | Sources or reference |
|---|---|---|
AFP111 | F + λ- rpoS396(Am) rph-1 ΔpflAB::Cam ldhA::Kan ptsG | |
DH5α | F-φ80 lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rk-, mk-) supE44 λ-thi-1 gyrA96 relA1 phoA | TransGen Biotech |
BL21 | F- ompT, hsdSB (rB- mB-), gal, dcm | TransGen Biotech |
Tang1505 | AFP111/pTrc99A | This study |
Tang1534 | AFP111/pTrc-cra | This study |
Tang1535 | AFP111/pTrc-cra with mutation at R57K | This study |
Tang1536 | AFP111/pTrc-cra with mutation at G59N + T76Y | This study |
Tang1537 | AFP111/pTrc-cra with mutation at G59Q | This study |
Tang1538 | AFP111/pTrc-cra with mutation at A58T + R149I | This study |
Tang1539 | AFP111/pTrc-cra with mutation at A58G + S75H | This study |
Tang1540 | AFP111/pTrc-cra with mutation at R60Q + D148I | This study |
Tang1541 | AFP111/pTrc-cra with mutation at R57K + A58G + G59Q + R60Q + S75H + T76Y + D148I + R149I | This study |
Tang1542 | AFP111/pTrc-cra with mutation at R57K + A58T + G59Q + R60Q + S75H + T76Y + D148I + R149I | This study |
Tang1543 | AFP111/pTrc-cra with mutation at R57K + A58G + G59N + R60Q + S75H + T76Y + D148I + R149I | This study |
Tang1544 | AFP111/pTrc-cra with mutation at R57K + A58T + G59N + R60Q + S75H + T76Y + D148I + R149I | This study |
Plasmids | ||
pTrc99A | ApR, pBR322 ori, trc promoter, lacIq | Invitrogen |
pET28a | KanR, pBR322 ori, lacIq, T7 promoter | Novagene |
pTrc-Cra | pTrc99A carrying cra gene | This study |
pET-Cra | pET28a carrying cra gene | This study |
pET-CGQ | pET28a carrying cra gene with mutation at R57K + A58G + G59Q + R60Q + S75H + T76Y + D148I + R149I | This study |
pET-CTN | pET28a carrying cra gene with mutation at R57K + A58T + G59N + R60Q + S75H + T76Y + D148I + R149I | This study |
