Figure 1

LRBA-null Hosts show Compromised Allogeneic, miHAg Mismatched, Missing Self and Xenogeneic BM Graft Rejection.

(A) Equal number of Balb/c (H2^d) and syngeneic 129Sv (H2^b) BM cells were mixed and injected into lethally irradiated WT and LRBA-null (Null) mice. Contour plot and scatter plot indicating presence of circulating allogeneic donor cells 7 day post BM transplantation are shown. (B) Lethally irradiated WT and Null mice received equal number of C57BL6 (CD45.1) miHAg mismatched and syngeneic 129Sv (CD45.2) BM cells. Thirty days post-BM transplantation splenocytes were analysed for presence of Ly5.1 and Ly5.2 cells by flow cytometry. (C) Equal number of TAP2^−/−β2m^−/− (missing self) BM cells stained with CFSE and 129Sv BM cells stained with DDAO were mixed and injected into lethally irradiated WT or Null hosts. Mice were bled and analysed for presence of TAP2^−/−β2m^−/− BM cells after 18 hrs and data is presented as contour and Box-and-whisker plot. (D) Sub-lethally (550cGy) or lethally irradiated WT or Null hosts received CFSE labeled human BM cells. After 18 hrs mice were bled and analysed for presence of CFSE⁺ human cells by flow cytometry. Results shown in A are representative of 3 independent experiments with n = 3–4 mice per group. Results shown in B, C and D are representative of two independent experiments with n = 3–4 animals per group. *p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001.