Table 2 Sphingolipid composition of patients with AERD and ATA before and after the ASA-BPT.

From: Exploration of the Sphingolipid Metabolite, Sphingosine-1-phosphate and Sphingosine, as Novel Biomarkers for Aspirin-exacerbated Respiratory Disease

Type of sample

SL metabolites

Before

P value† AERD vs ATA

After

P value† AERD vs ATA

AERD

ATA

AERD (N = 45)

ATA (N = 45)

AERD (N = 45)

ATA (N = 45)

P value‡ before vs after

P value‡ before vs after

Serum (ng/mL)

S1P

101.86 ± 23.48

85.69 ± 20.76

0.001

112.62 ± 20.50

95.71 ± 16.64

<0.001

0.022

0.026

 

Sphingosine

4.23 ± 6.66

1.47 ± 1.13

0.009

3.24 ± 3.53

1.58 ± 1.21

0.004

0.053

0.286

 

C16:0 Ceramide

56.48 ± 47.92

46.02 ± 40.02

0.391

41.72 ± 35.71

39.39 ± 31.36

0.773

0.129

0.347

 

C18:0 Ceramide

596.9 ± 426.14

561.39 ± 458.85

0.753

459.31 ± 471.8

698.98 ± 690.07

0.091

0.189

0.258

 

C18:1 Ceramide

26.16 ± 23.32

17.89 ± 14.33

0.096

19.7 ± 16.23

21.45 ± 14.5

0.600

0.182

0.284

 

C20:0 Ceramide

431.63 ± 289.19

425.25 ± 334.92

0.856

401.64 ± 437.73

528.77 ± 451.32

0.323

0.725

0.236

 

C24:0 Ceramide

43487.36 ± 68722.91

46939.51 ± 65163.38

0.872

28827.32 ± 31166.87

45757.38 ± 46134.42

0.111

0.135

0.905

 

C24:1 Ceramide

16428.46 ± 22695.26

14179.54 ± 19020.02

0.469

13063.48 ± 17229.03

15799.6 ± 15430.6

0.565

0.225

0.443

 

SM (d18:0/16:0)

634689.34 ± 834083.29

574655.49 ± 496300.08

0.478

418167.27 ± 373508.9

589256.12 ± 543663.91

0.243

0.050

0.887

 

SM (d18:0/18:0)

117147.07 ± 100604.79

119072.53 ± 112764.54

0.758

87509.14 ± 57956.94

118776.79 ± 93683.63

0.235

0.035

0.990

 

SM (d18:0/18:1)

288429.15 ± 334236.26

251856.35 ± 230136.98

0.346

196502.31 ± 162071.74

254202.24 ± 186918.08

0.348

0.038

0.962

 

SM (d18:0/24:0)

229334.6 ± 239649.44

192279.45 ± 160687.77

0.237

156231.31 ± 129139.24

199692.37 ± 163019.24

0.417

0.028

0.813

 

SM (d18:0/24:1)

401790.38 ± 393446.33

300081.46 ± 261921.51

0.170

266693.65 ± 207376.38

318932.87 ± 278792.52

0.469

0.013

0.745

 

Lyso SM

1348.72 ± 1637.08

1040.93 ± 849.71

0.239

908.74 ± 716.62

1135.74 ± 955.2

0.341

0.060

0.606

Urine*

S1P

34.19 ± 9.86

34.66 ± 12.43

0.629

41.19 ± 1.19 1

40.32 ± 0.32 6

0.726

0.001

0.005

(pmol/mg Cr)

Sphingosine

48.72 ± 21.58

31.29 ± 13.66

<0.001

50.11 ± 0.11 8

30.16 ± 0.16 1

<0.001

0.532

0.264

 

C14:0 Ceramide

154.67 ± 54.67 1

166.96 ± 66.96 9

0.596

144.98 ± 44.98

242.02 ± 42.02 6

0.355

0.424

0.684

 

C16:0 Ceramide

2807.85 ± 3699.32

3304.29 ± 5801.60

0.446

2932.36 ± 3180.57

5292.69 ± 9283.27

0.342

0.335

0.234

 

C18:0 Ceramide

399.31 ± 38.46

471.51 ± 86.96

0.524

420.10 ± 95.74

839.06 ± 517.83

0.274

0.437

0.151

 

C20:0 Ceramide

650.46 ± 16.87

601.17 ± 305.52

0.725

726.95 ± 49.77

916.92 ± 80.26

0.534

0.275

0.178

 

C24:0 Ceramide

1861.21 ± 1965.82

2214.40 ± 3925.29

0.340

1962.82 ± 1545.23

3864.77 ± 7114.12

0.193

0.281

0.137

 

C24:1 Ceramide

343.32 ± 95.64

726.78 ± 381.57

0.058

378.00 ± 60.49

1215.88 ± 3931.01

0.250

0.239

0.314

 

Sphinganine

1184.94 ± 03.38

464.92 ± 0.14

0.072

1255.57 ± 2057.80

725.73 ± 1036.23

0.214

0.513

0.049

 

SM (d18:0/16:0)

102343.59 ± 64388.97

97934.29 ± 92854.80

0.662

105220.51 ± 58546.58

111780.95 ± 85951.83

0.593

0.457

0.144

 

SM (d18:0/18:0)

24703.85 ± 24562.57

25473.65 ± 32979.63

0.259

23060.26 ± 16354.33

35243.73 ± 47199.35

0.216

0.900

0.143

 

SM (d18:0/18:1)

1672.54 ± 1431.48

2409.25 ± 4176.42

0.221

1634.46 ± 1088.91

4708.95 ± 14959.42

0.248

0.673

0.248

 

SM (d18:0/24:0)

82282.05 ± 65291.75

75552.38 ± 69356.22

0.719

79694.87 ± 43651.90

96132.00 ± 80454.76

0.351

0.661

0.074

 

SM (d18:0/24:1)

40989.74 ± 38049.69

40254.86 ± 46033.41

0.450

38362.31 ± 24894.45

53031.24 ± 66543.38

0.224

0.863

0.115

  1. AERD, aspirin-exacerbated respiratory disease; ATA, aspirin- tolerant asthma;, SM, sphingomyelin; Cr, creatinine.
  2. *C14 Ceramide and sphinganine were not detected in human serum samples.
  3. †P value was calculated by GLM, controlling for sex and age. Values in bold indicate significant P value.
  4. ‡P value was calculated using a paired t-test.
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