Figure 1

(A) Scheme of a classic four-step CHA pathway. Complementarity between numbered domains is denoted by an asterisk (*). (B) Schematic table showing average operating temperatures of an ideal regular CHA (Ideal), a structure ruined regular CHA (Reality), a high temperature CHA (HT-CHA), and an organic amide promoted HT-CHA (OHT-CHA). (C) Scheme of coupling OHT-CHA with LAMP to achieve ultra-sensitive, reliable and compatible gene diagnosis.