Figure 2

Expression dynamics of chicken stage X blastoderm-specific HSP30 subfamily.
Quantitative expression analysis of blastoderm-specific chicken HSP30 subfamily during intrauterine and early development (a) and egg storage (b). Real-time PCR was conducted in triplicate and normalised to expression of GAPDH and ACTB. Significant differences between groups are indicated by different letters. Error bars indicate the SE of triplicate analyses. EGK, Eyal-Giladi and Kochav stage; 4 h, 4-h-incubated embryo at 37.5 °C; d, days of storage at 16 °C.