Figure 5 | Scientific Reports

Figure 5

From: The avian-specific small heat shock protein HSP25 is a constitutive protector against environmental stresses during blastoderm dormancy

Figure 5

Anti-apoptotic function of HSP25 in mitomycin C-treated chicken blastoderm cells.

(a) Annexin V/PI analysis by flow cytometry after HSP25 knockdown followed by mitomycin C treatment (0, 30, or 60 μM). Quantitative analysis of double-negative (b) and double-positive (c) cells, indicating live and apoptotic cells, respectively. (d) Relative expression analysis of anti-apoptotic genes after HSP25 knockdown followed by 60 μM mitomycin C treatment. Non-complementary sequences in the chicken genome were used as a control. Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. ###P < 0.001 and ##P < 0.01 significance of mitomycin C treatment (30, or 60 μM) compared to 0 μM. Significant differences between control and HSP25 knockdown are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate the SE of triplicate analyses.

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