Figure 5
From: Glycation potentiates neurodegeneration in models of Huntington’s disease
Knockdown of Glo1 or Tpi impairs development and reduces lifespan in flies. RNAi silencing of Glo1 (a) or Tpi (b) caused a reduction in the percentage of flies emerging from the pupal case. Flies carrying a single copy of the driver (elavGAL4) are shown as a control. Data in panels (a,b) are mean ± SEM. Survival rate was evaluated in flies with pan-neuronal knockdown of Glo1 (c) or Tpi (d) in mutant HTT backgrounds (n = 100–150 flies per genotype). 3M + Htt93Q and GFP + Htt93Q were used in experiments as titration controls for GloI and Tpi silencing lines, respectively. Htt93Q + Glo1 RNAi (mean = 6); Htt93Q + 3M (mean = 12); Htt93Q + Tpi a RNAi (mean = 12); Htt93Q + Tpi b RNAi (mean = 11.5); Htt93Q + GFP (mean = 13). *p < 0.05, **p < 0.01, ****p < 0.0001; for (a,b) Ordinary one-way ANOVA with Newman-Keuls multiple comparisons test; for (c,d) Log-rank (Mantel-Cox) test.
