Figure 5

Dynamics of behavioral responses (withdrawal) amplitudes in 4 groups of snails after aversive conditioning.
(A) Protocol of the context conditioning experiment; open and closed blocks represent days of experiment; T0-4 – testing of tentacle withdrawal amplitude in Context 1 (ball), and Context 2 (glass). (B) averaged results of behavioral testing demonstrating that under blockade of serotonergic system (G3), memory exists on the first test (T2), diminishes next day (T3), and disappears at the third test after injection of the neurotoxin (T4). *p < 0.05, **p < 0.01; ***p < 0.001 here and on other figures. G1 – Reminder+anisomycin injection; G2 – Reminder+5,7-DiHT+anisomycin injections; G3 – Reminder+5,7-DiHT injection; G4 – Reminder+saline injection. All tests performed in Context 2 (glass) are shown as black bars for all groups, tests in Context 1 (ball) are shown with different patterns for each group.