Figure 7
Averaged changes in behavioral responses to food presentation in terrestrial snails after association of food presentation with electric shock (training).
(A) Protocol of food-aversion conditioning experiment with injection after test 1 (T1) of 5,7-DiHT to G3, injection before the T2 of saline to G1 (control), anisomycin (injected only once) to G2, mianserin to G4. Mianserin was additionally injected repeatedly after each following test (T3-T7). Each test was considered as a Reminder. (B) Averaged results of behavioral testing of latency of feeding responses demonstrating that all snails significantly increased latency of responses after training (T1), control snails (G1) demonstrated presence of memory throughout the experiment (T2-7), while the Reminder+anisomycine group (G2) demonstrated absence of memory (T4-7). Snails under blockade of serotonergic system (G3) demonstrated existence of memory on the first test, significant (p < 0.001) decrease of memory at T3-T7 tests after injection of neurotoxin relative T1, but still a significant difference from the initial (T0) score at T3-7 tests, suggesting maintenance of a significantly decreased memory. The group of snails (G4) injected with mianserin preserved memory similar to the control group.
