Figure 8
Morphology of input-output connections of serotonergic neurons in terrestrial snails.
(A) Schematic representation of the position and sizes of serotonergic neurons of the rostral pedal cluster. Identifiable by size, position and electrophysiological characteristics neurons are numbered. CPdC –cerebro-pedal commissure; PlPdC – pleuro-pedal commissure; St – statocyst. (B) 10 micrometer unstained section of a part of pedal ganglia containing the Pd4 neuron (largest soma) from snails injected 2 months before with 5,7-DiHT. The deep brown color in cytoplasm is due to the intracellularly oxidized 5,7-DiHT accumulated in lysosomes that act as the waste disposal system of the cell in this case. (C–F) Location of the cell bodies and terminal branching zones revealed by retrograde staining of cerebral and pedal ganglia with Neurobiotin via the left cerebro-pedal connective. (C) Schematic representation of cerebral ganglion neural somata clusters sending axons to the CPdC; (D) Schematic representation of the neuropil areas (NA) in the cerebral ganglion where input neurites from pedal ganglia terminate; (E) Schematic representation of neuropil areas in the pedal ganglion where input neurites from cerebral ganglia terminate; (F) Schematic representation of pedal ganglion neural somata clusters sending axons to the CPdC.
