Figure 3
From: Internal epitope tagging informed by relative lack of sequence conservation
Epitope tag integration events transmitted through the germline.
(a) An alignment of 5′ junction fragments between tcf21 and V5 tag integration events transmitted through the germline. PAM sequence is in bold, sgRNA target is highlighted in cyan, the homology arm present in the HDR oligo is in magenta, sequence of the V5 tag is in blue, and mismatches are in red. (b) DNA and protein sequence of the recovered tcf 21-V5 allele, identical to that shown as inx12b in (a). Red indicates nucleotide and/or amino acid changes compared to the expected sequence. (c) An alignment of 5′ junction fragments between tbx18 and V5 tag integration events transmitted through the germline.
