Figure 3
Low pH leads to a decrease in the anabolic protein aggrecan.
(a) Gene expression of AGC relative to MRPL19 following culture of NP cells (n = 3) at pH 7.4, 7.1, 6.8 and 6.5 for 7 days and normalised to gene expression at pH 7.4. (b) Western blot for aggrecan protein expression following culture of NP cells (n = 3) at pH 7.4, 7.1 and 6.5 for 7 days and normalised to GAPDH. *Indicate p ≤ 0.05. (c) Immunofluorescence for AGC protein (green) and cell nuclei (DAPI; blue) of NP cells cultured at pH 7.4, 7.1 and 6.5.
