Figure 5
From: Product release is rate-limiting for catalytic processing by the Dengue virus protease
Time course of fluorescence changes.
Changes in fluorescence recorded upon the addition of Ac-LKRR-pNa substrate (25 μM) for both the glycine-linked and unlinked systems, for their wild-type (WT) and Ser83Trp mutant forms. Fits to the data are shown in black (a) Unlinked enzymes (b) Glycine-linked enzymes.
