Table 4 Comparison of steady state (kcat, Km, kcat/Km) and pre-steady state (kobs, k1, k2) kinetic parameters for wild-type and Ser83Trp mutant forms of the glycine-linked and unlinked NS2BH-NS3pro complexes, using the substrate Ac-LKRR-pNa at 37 °C, pH 8.5.
From: Product release is rate-limiting for catalytic processing by the Dengue virus protease
Km (μM) | kcat (s−1) | kcat/Km (M−1s−1) | kobs (s−1) | k1 (s−1) | k2 (s−1) | |
|---|---|---|---|---|---|---|
Glycine-linked WT | 282 ± 22 | 0.34 ± 0.01 | 1191 ± 104 | 0.82 ± 0.31 | 12 ± 1 | — |
Glycine-linked S83W | 624 ± 69 | 1.17 ± 0.09 | 1880 ± 252 | 0.78 ± 0.43 | 15 ± 3 | 350 ± 23 |
Unlinked WT | 197 ± 21 | 0.46 ± 0.02 | 2345 ± 276 | 0.88 ± 0.23 | 112 ± 11 | — |
Unlinked S83W | 215 ± 17 | 0.47 ± 0.01 | 2180 ± 190 | 0.58 ± 0.20 | 52 ± 2 | 210 ± 32 |
