Figure 4
From: Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction
Effects of combining trehalose and TMAC on EXPAR.
(A) Typical real-time amplification plot of EXPAR performed with positive target (solid line) and no-target control (NTC, dotted lines) at 0 M of trehalose +0 mM of TMAC, 0.4 M trehalose, 40 mM TMAC and 0.4 M trehalose +40 mM TMAC. (B) Time lag between targeted and NTC with or without TMAC and trehalose. (C) Top: Typical gel electrophoresis image of corresponding EXPAR products. Full-length gel image provided in Supplementary Figure S8. Bottom: Bar graph of average ssDNA band intensities normalized to NTC without trehalose and TMAC. Error bars represent SD, n = 2.
