Table 1 Comparative analyses of various chromosomal HP0539/cagL deletion mutants.

H. pylori strains	CagL amount	CagL secretion^a	CagI amount	CagH amount	CagA amount	IL-8 induction in AGS cells	CagA translocation
SU2 wt	++	++	++	++	++	++	++
cagL::aphA3	−	−	+	++	++	−	n.d.
CagL^ΔTSPSA	++	++	++	++	++	++	++
CagL^ΔNNEM	++	++	++	++	++	++	++
CagL^ΔRGD	++	++	++	++	++	++	++
CagL^ΔELFFI	+	+	+	++	++	+	−
CagL^ΔSEDVI	+	+	+	++	++	++	+
CagL^ΔFKTYM	+	+	+	++	++	+	−
CagL^ΔLDQNT	−	−	−	++	++	−	n.d.
CagL^ΔAEQQC	+	++	++	++	++	++	+
CagL^ΔGISDK	−	−	+	++	++	+	n.d.
CagL^ΔTASLI	+(+)	++	++	++	++	++	++
CagL^ΔRGDΔTASLI	−	+	+	++	++	++	+
CagL^ΔTSPSAΔRGD	−	+	+	++	++	++	++
CagL^T¹^70K	++	+	+	++	++	++	++

The plus and minus signs in each column indicate: [++], [+(+)] normal to slight reduction, [+] clear reduction, and [−] strong reduction of the respective protein or function (column header) in comparison with the parental strain (reference, SU2 wild type [wt]).
^aThe short term “CagL secretion” indicates that CagL was detected in the outer fraction of the bacteria which includes surface-associated material; CagL has been detected before by others on the bacterial surface^27,24. n.d. = below detection level.

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