Figure 3
From: Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer
Metabolomic analysis for identification of metabolic effects of oncogene KRAS.
(A,B) KRAS knockdown DLD1 cells and control cells were cultured at 3 × 106 cells per 10-cm dish in 10 ml complete medium; the medium was replaced the following day with glucose(−) and glutamine(−) medium supplemented with 10% dialyzed fetal bovine serum. Glucose (10 mM) or glutamine (2 mM) was added, respectively. For hypoxia treatment, cells were incubated for 24 h with 1% O2. Principal component analysis (A) and hierarchical cluster analysis (B) performed under the indicated conditions at the indicated time points. The red circles indicate the groups that have similar principal component scores. Data for two independent experiments are presented.
