Figure 4
From: Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer
DLD1 cells retain TCA cycle activity under condition of glucose depletion.
DLD1 and HT29 cells were cultured at 3 × 106 cells per 10-cm dish in 10 ml complete medium; this medium was replaced the following day with glucose(−) and glutamine(−) medium supplemented with 10% dialyzed fetal bovine serum. Glucose (10 mM) or glutamine (2 mM) was added, respectively. Cells incubated for 24 h were analyzed. Data are presented as mean ± SD for two independent experiments.
