Figure 6
Selectively Down-regulation of TRPM2 in SCC9 Cell Lines Promoted Apoptosis of Cell and Altered the Expression of Related Protein.
(A) The number of apoptotic cells was measured after SCC9 cells were transfected with shRNAVec or shRNATRPM2 for 48 hours by staining with Annexin V-FITC and PI and with flow cytometry techniques. (B) Apoptotic cells were increased in SCC9 cells 48 hours after being transfected with shRNATRPM2. (control: 18 ± 1%; shRNAVec: 19 ± 1%; and shRNATRPM2: 57 ± 2%. n = 3). (C) Western blotting revealed that transfection of SCC9 cells with shRNATRPM2 for 48 hours significantly suppressed the expression of tumor-related protein p53, but increased the level of p21 expression. (D) Quantitative results for the Western blotting of each group (n = 3). **p < 0.01 compared with the control group.
