Figure 4

In silico GO and KEGG-pathway enrichment analysis of miRNAs detected in hAMSC-CM-derived exosomes.
(a) Go analysis indicated that ~40% of the targeted genes were associated with cellular components, including 24% with organelles. (b) GO molecular functions analysis resulted in 33% involved as binding molecules and 29% with catalytic activity. (c) GO terms associated with biological processes were more diversified, with 25% of the target molecules involved in metabolic processes, 21% in cellular processes, 13% in biological regulation, 9% in localization, 8% in developmental processes. (d) KEGG-pathway enrichment indicated ‘Pathways in cancer’ as the top-scoring pathway among 173 KEGG pathways. (e) qRT-PCR validation of miRNA expression in exosomes, and host cells (hAMSCs). The miRNA-validation experiments were repeated at least three times. *p < 0.05, **p < 0.01 and ***p < 0.001.