Figure 4 | Scientific Reports

Figure 4

From: Inhibition of PKR protects against H2O2-induced injury on neonatal cardiac myocytes by attenuating apoptosis and inflammation

Figure 4

PKR mediates NFκB activation signaling in H2O2 treated NCM.

Cultured NCM were left untreated or stimulated with 4 mM 2-AP for 30 minutes, followed by stimulation with H2O2 (50 μM) for 12 h. (A) Analysis of NFκB activation by EMSA; (B) Analysis of NFκB activation by TransAM p65 NFκB assay kit. (C) Real-time PCR analysis of TNF-α mRNA expression. (D) ELISA assay for detection the TNF-α release in medium. (E) Real-time PCR analysis of IL-6 mRNA expression. (F) ELISA assay for detection the IL-6 release in medium. The data are representative of three independent experiments. (**P < 0.01; *P < 0.05). Full length blots were shown in Supple Fig. 4.

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