Figure 1

TREM-2 expression is up-regulated in human acquired cholesteatoma.
(A) TREM-2 expression was estimated by quantitative real-time polymerase chain reaction. (B) Representative pictures of Immunohistochemical analysis in human acquired cholesteatoma and normal skin. TREM-2+ cells are depicted in brown. Panels are representative of 11 samples of human acquired cholesteatoma and 11 samples of normal skin. Images were acquired with a biomicroscope (CX21, Olympus, Japan) and images of 100× were acquired with an oil immersion objective. *Represents a TREM-2+ cell. (C) TREM-2+ cells were counted in 5 independent 40× fields and the average values were calculated. Then, correlation analysis was applied between the amount of TREM-2+ cells and the level of bone destruction (n = 11).