Figure 1 | Scientific Reports

Figure 1

From: A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield

Figure 1

Reduction of rabbit and goat IgG with thiol reductants.

Rabbit IgG (Panel a) and goat IgG (Panel b) were treated with various concentrations of β-ME for 1 h at 37 °C and subjected to SDS-PAGE under non-reducing condition. Rabbit IgG was treated with various concentration of sodium sulphite and 2.5 mM DTNB for 2 h at 37 °C, dialysed and subjected to SDS-PAGE under non-reducing condition (Panel c). M, marker proteins. Full length gel images are presented in Supplementary Fig. S1.

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