Figure 5
From: A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield
Preparation of BsAbs using anti-α-LA and anti-HRP antibodies, affinity purified from the sera of immunized rabbits.
The immuno affinity purified anti-α-LA and anti-HRP antibodies were reduced separately with 10 mM β-ME for 1 h and mixed in 1:1 ratio in presence of 0.1% (w/v) SDS or absence of the detergent and dialysed against PBS, pH 7.4. Formation of the BsAbs was determined by ELISA (Panel a). Results are expressed as mean ± SD of three independent experiments. The preparations thus obtained were subjected to non-reducing SDS-PAGE [Panel b, IgG reduced to half molecules (lane 1), molecular weight markers (lane 2) and fraction after dialysis (lane 3)]. Full length gel images are presented in Supplementary Fig. S1.
