Figure 6
From: A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield
Preparation of BsAbs using the IEC fractions isolated from sera of rabbits and commercial goat IEC fraction as well as purification of rabbit anti-α-LA: anti-HRP BsAbs by twin affinity chromatography.
Panel a: Rabbit anti-α-LA: rabbit anti-HRP BsAbs were prepared essentially as described from the immuno affinity fractions (legend to Fig. 5), and assayed for the presence of BsAbs by ELISA. Panel b: BsAbs recognizing HRP and HSA were prepared using goat anti-HSA and anti-HRP antibodies, in absence (
) or presence of SDS (
). BsAbs were also prepared from goat anti-HSA and rabbit anti-HRP in absence (
) or presence of SDS (
) and assayed by ELISA. Activity of the BsAb prepared from the IEC fractions of rabbit anti-α-LA and anti-HRP antibodies before (Panel c) and after twin affinity purification (Panel d) in which fifty milligrams each of rabbit anti-α-LA and anti-HRP IEC fractions were subjected to reduction and oxidation in presence of 0.1% (w/v) SDS to obtain BsAbs and activity assayed by ELISA. Data are presented as the mean ± SD of three independent experiments.
