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Figure 1

From: Pharmacological and genetic reappraisals of protease and oxidative stress pathways in a mouse model of obstructive lung diseases

Figure 1

Airway mucus obstruction and inflammation of low-mortality airway-specific βENaC-Tg mice with C57/BL6J background.

(a) Mortality of βENaC-Tg mice backcrossed with C57/BL6J mice for three generations. (b) Pulmonary mRNA expression of Scnn1b (βENaC) gene in WT (n = 14) and C57/BL6J-βENaC-Tg (n = 17) mice quantified by Q-RT-PCR. (c) Mucus plug in BALF of WT and C57/BL6J-βENaC-Tg mice. (d–g) Representative data (n = 4–5) of PAS and alcian blue-stained section. Mucus plug observed in C57/BL6J-βENaC-Tg mice (f,g), but not in WT mice (d,e). Scale bars, 1,000 μm (d,f) and 100 μm (e,g). (h–k) Muc5ac (h), fucose (i) and KC (k) concentration in BALF of WT (n = 9) and C57/BL6J-βENaC-Tg WT (n = 11) mice. Pulmonary Gob5 gene expression (j) in WT (n = 14) and C57/BL6J-βENaC-Tg (n = 17) mice. (l) Inflammatory cell count in BALF of WT (n = 4) and C57/BL6J-βENaC-Tg WT (n = 5) mice. *p < 0.05, **p < 0.01, ***p < 0.001, versus WT; Student’s t test.

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