Figure 1
From: Hephaestin and ceruloplasmin facilitate iron metabolism in the mouse kidney
Iron staining and ferritin protein expression in the renal medulla and cortex.
Iron levels and distribution were determined by Perls’ Prussian blue staining of kidney sections. Representative photomicrographs of the medulla (A) and cortex (B) are shown. Strongest iron deposition was observed in the medulla of Heph/Cp KO mice. Ferritin protein expression was assessed by Western blotting in the renal medulla (C) and renal cortex (D) of WT, Heph/Cp KO, Cp KO, and Heph KO mice. Tubulin served as a loading control. Levels of ferritin protein were quantified by densitometry and these results are shown in parts E (medulla) and F (cortex). Ferritin expression was normalized to tubulin, and then presented as a ratio of ferritin expression in WT mice. Values are presented as the mean ± SEM, n = 4–5 mice per genotype. Within each panel, bars without common letters are significantly different, P < 0.05.
