Figure 2
From: Hephaestin and ceruloplasmin facilitate iron metabolism in the mouse kidney
CP, HEPH, and FPN1 protein expression in the renal medulla and cortex.
Western blotting was used to investigate CP (A,B,E,F), HEPH (A,B,G,H) and FPN1 (C,D,I,J) expression in the renal medulla (left hand panels) and cortex (right hand panels) of WT, Heph/Cp KO, Cp KO and Heph KO mice. Tubulin served as a loading control. For the blots of FPN1, the samples were not heated prior to loading onto the gels. Levels of Cp (E,F), Heph (G,H) and FPN1 (I,J) protein were quantified by densitometry. All levels were normalized to tubulin. Values are presented as the mean ± SEM, n = 4–5 mice per group. Within each panel, bars without common letters are significantly different, P < 0.05.
