Figure 3
From: Hephaestin and ceruloplasmin facilitate iron metabolism in the mouse kidney
DMT1 (IRE) and TFR1 protein expression in the renal medulla and cortex.
Western blotting was used to assess the levels of DMT1 (A–D) and TFR1 (A,B,E,F) protein expression in the renal medulla (left hand panels) and cortex (right hand panels) of WT, Heph/Cp KO, Cp KO, and Heph KO mice. Tubulin served as a loading control. For DMT1 blots, 150 μg of protein was loaded per well for renal medulla and 40 μg of protein was loaded per well for renal cortex. Densitometric analysis of the western blots is shown for DMT1 (C,D) and TFR1 (E,F) protein. All levels were normalized to tubulin. Values are presented as mean ± SEM, n = 4–5 mice per group. Within each panel, bars without common letters are significantly different, P < 0.05.
