Figure 3

Metabolic incorporation patterns of different metabolite species from quantitative ratiometric images.

(a) Ratiometric images of new protein synthesis (C-D 2133 cm⁻¹) over old proteins (CH₃ 2940 cm⁻¹) in both dentate gyrus and CA1 regions of hippocampus. Protein synthesis is highly active in the neuronal cell body particularly the nucleoli (arrows). (b) Ratiometric images of new choline phospholipid synthesis (alkyne 2140 cm⁻¹) over old lipids (CH₂ 2845 cm⁻¹) in both dentate gyrus and CA1 regions of hippocampus. Choline is enriched in soma structures of selected cells (arrows). (c) Ratiometric images of fatty acid incorporation and new lipid synthesis (C-D 2105 cm⁻¹) over old lipids (CH₂ 2845 cm⁻¹) in both dentate gyrus and CA1 regions of hippocampus. Palmitic acid is evenly incorporated in the hippocampal tissues. Scale bar: 40 μm.