Figure 5

Effect of H₂S and Cys on the expression of AOX genes and the activity of TP, CP and AP in Cd²⁺ stress.

(a–c) The expression of AOX1A, AOX1C and AOX2. 7-d-old WT seedlings were grown on 1/2 MS agar plates supplied with 50 μM Cd²⁺, 50 μM NaHS, 1 mM Cys, 50 μM Cd²⁺ plus 50 μM NaHS, and 50 μM Cd²⁺ plus 1 mM Cys for 3 h, respectively. (d,e) Changes in TP, CP and AP activity in various Cd²⁺ concentrations for 5 d in WT. (f–l) AP activity in WT and mutants. 7-d-old Arabidopsis seedlings were grown on 1/2 MS agar plates treated with 50 μM Cd²⁺, 50 μM Cd²⁺ plus 50 μM NaHS and 50 μM Cd²⁺ plus 1 mM Cys for 5 d. Mean values and SE were calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan’s multiple range tests).