Figure 5: MiR-291b-3p elevates the expression of PTEN. | Scientific Reports

Figure 5: MiR-291b-3p elevates the expression of PTEN.

From: Hepatic MiR-291b-3p Mediated Glucose Metabolism by Directly Targeting p65 to Upregulate PTEN Expression

Figure 5

(A) The mRNA level of AMPK in the liver of fasted mice. (B) The hepatic phosphorylation level of AMPK in the C57 mice injected with compound c, an AMPK inhibitor, at a dose of 2 mg/kg for 3 days. (C) The glycogen level in the liver of C57 mice transfected with Ad-NC and Ad-291m, and injected with compound C. (D) The mRNA levels of G6Pase, PGC1-α and PEPCK in the liver of C57 mice transfected with Ad-NC and Ad-291m, and injected with compound C. (E) The hepatic level of PTEN protein in the liver of HFD-fed mice on 7 days after injection of Ad-miR-291i or Ad-NC. (F) The hepatic expression of PTEN protein in the liver of C57BL/6 J mice on 7 days after injection of Ad-miR-291i or Ad-NC. (G) The protein level of PTEN in the NCTC1469 cells transfected with 291i or NC. (H) The expression of PTEN protein in the NCTC1469 cells transfected with 291 m or NC. Full-length blots/gels are presented in Supplementary Figure 5 and cropping lines are indicated in red color. Data are expressed as mean ± SEM (n = 5). *P < 0.05; **P < 0.01 vs. control group.

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