Figure 2: Propidium iodide (PI) staining of embryo-derived EVs in embryo culture medium.

(a) Representative FSC-SSC dot plot shows the size distribution of EVs in embryo culture medium. (b) Representative dot plot shows the autofluorescence of embryo culture medium in FL2 channel (embryo culture medium + 4% formaldehyde solution +PI, without EVs. (c) Representative dot plot shows the PI fluorescence of 4% formaldehyde fixed embryo-derived EVs in embryo culture medium.