Figure 6: Expression of viral genes in an uninfected population of cells co-cultured with influenza infected cells.
From: Influenza virus exploits tunneling nanotubes for cell-to-cell spread
Cells of the R- and R/G- quadrant were cultured for up to 36 h post-sort and assays conducted as denoted. (a) RT-PCR analysis in cells of the R/G and R quadrant showing fold-increase in expression of viral genes. (b) Immunofluorescence-based infectivity assay showing GFP fluorescence in MDCK cells overlaid with cells from the R/G and R quadrants.
