Figure 4: IRTKS and IRSp53 double knockout leads to embryonic lethality. | Scientific Reports

Figure 4: IRTKS and IRSp53 double knockout leads to embryonic lethality.

From: Redundant functions of I-BAR family members, IRSp53 and IRTKS, are essential for embryonic development

Figure 4

(A) Western blot of IRSp53 and IRTKS in total protein lysates of embryonic fibroblasts. The images were cropped for clarity of presentation. Full-length blots are presented in supplementary figure S5A. (B) Genotype ratios of IRSp53+/−; IRTKS−/− intercrosses at different embryonic stages and weaning. 7–13 litters were analysed for each stage. n.d.* resorbed embryos; undetermined genotypes. (C) Examples of E10.5 IRTKS−/− embryos that are wild-type, heterozygous or null for IRSp53. Arrowheads point to heart malformations in the double knockout (dKO) embryos. (D) Embryonic hearts dissected at E14.5. Misshapen right ventricles were observed in the dKO hearts (arrowheads). (E) Histological analysis of embryonic hearts at E14.5 in IRSp53+/+; IRTKS−/− and IRSp53−/−; IRTKS−/− embryos. H&E staining of transverse sections (left panels) revealed abnormalities in the membraneous ventricular septum (boxed) of dKO hearts, similar to phenotype observed in IRSp53−/− single mutants. Right panels show boxed region at higher magnification. Scale: 200 μm.

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