Figure 5: KSHV miRNAs repress the levels of erythropoietin-receptor (EPOR) mRNA and reduce erythropoietin-dependent STAT5 activation in endothelial cells.
From: Viral microRNAs Target a Gene Network, Inhibit STAT Activation, and Suppress Interferon Responses
(A) HUVECs were transfected with individual KSHV miRNA mimics along with control miRNAs and the levels of EPOR mRNA were measured 48 hpt by qRT-PCR. B. HUVECs were transfected with KSHV miRNAs (miR-K6-3, miR-K6-5, miR-K10b or miR-K12) or miR-Neg. At 48 hpt, transfected cells were mock-treated or treated with EPO for 10 minutes. Equal amounts of protein were probed for pY694-STAT5, JAK2 and total STAT5. “M” denotes the protein size marker loaded in the center lane. Relative levels of various proteins were normalized to actin and quantified below. Full-length blots are in Figure S3.
