Figure 2: Epinephrine and acetylcholine affected SCO in rat cardiomyocyte cultures. | Scientific Reports

Figure 2: Epinephrine and acetylcholine affected SCO in rat cardiomyocyte cultures.

From: Modification of distinct ion channels differentially modulates Ca2+ dynamics in primary cultured rat ventricular cardiomyocytes

Figure 2

(A) Representative traces of epinephrine-induced alterations in Ca2+ dynamics. (B) Representative traces of acetylcholine-induced alterations in Ca2+ dynamics. (C) Epinephrine concentration-dependently increased SCO frequency with an EC50 value of 1.43 nM (0.75–2.75 nM, 95% CI). Acetylcholine concentration-dependently decreased SCO frequency with an IC50 value of 2.10 nM (1.13–3.89 nM, 95% CI). (D) Both epinephrine and acetylcholine stimulated SCO amplitude. The EC50 values for epinephrine and acetylcholine stimulating SCO amplitude was 0.86 nM (0.33–2.20 nM, 95% CI) and 3.77 nM (0.73–19.4 nM, 95% CI), respectively. (E) Epinephrine concentration-dependently decreased the SCO duration with an IC50 value of 0.92 nM (0.79–1.06 nM, 95% CI). Acetylcholine was without effect on the SCO duration. The experiments were repeated in three independent cultures each with four replicates.

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