Figure 4: Comparison of eDNA content between untreated biofilms and those formed in the presence of low phage concentrations. | Scientific Reports

Figure 4: Comparison of eDNA content between untreated biofilms and those formed in the presence of low phage concentrations.

From: Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus

Figure 4

(A) Agarose gel visualization of eDNA prepared from untreated biofilms (lanes 1 and 2) and biofilms formed in the presence of phiIPLA-RODI (MOI of 10−5) (lanes 3 and 4). (B) DNAse treatment of 24 hour-biofilms developed without phage or with an MOI of 10−5. Black and grey bars represent biofilms incubated without or with 200 μg/ml DNAse I for one hour at 37 °C. Values represent the average and standard deviation of four independent biological replicates. *P-values < 0.05.

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