Table 2 Confirmation by RT-qPCR of the dysregulation for selected genes identified by RNA-seq analysis.

From: Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus

Gene

Gene product

FC (RT-qPCR)

FC (RNA-seq)

atl

Bifunctional autolysin

31.92 ± 18.84

5.42

rsh

GTP pyrophosphokinase

33.90 ± 17.22

4.91

relP

GTP pyrophosphokinase

23.54 ± 8.95

2.21

clpC

ATP-dependent Clp protease, ATP-binding subunit

−4.96 ± 0.99

−22.97

clpB

ATP-dependent Clp protease, ATP-binding subunit

−28.03 ± 9.39

−112.83

crtP

Diapolycopene oxygenase

25.59 ± 14.78

3.34

dltA

D-alanine—poly(phosphoribitol) ligase subunit 1

20.21 ± 11.85

3.69

sigB

RNA polymerase sigma factor

16.13 ± 5.73

2.56

nuc

Thermonuclease

19.20 ± 11.24

5.72

icaA

Poly-beta-1,6-N-acetyl-D-glucosamine synthase

4.06 ± 0.76

3.16