Figure 6: Chrysophanol inhibited MNU-induced expression of apoptosis-related proteins.

PARP, Bax, caspase-3, and p-c-Jun protein levels in retinal homogenates were evaluated using western blot. All the Western blotting experiments were performed under the same conditions. After transferring the blots onto nitrocellulose membranes, we immediately cropped the targeted blots according to referenced indicating markers (Supplementary Fig. S1), and then targeted proteins were immunoblotted with its specific monoclonal antibody. The quantification values were presented as the mean ± SEM of at least 3 retinas from each group. Representative bands from 2 independent experiments for each group: the control (C1, C2), vehicle-treated (V1, V2), and chrysophanol-treated (D1, D2) groups. C, CTRL: control; V: vehicle; D: chrysophanol-treated; N: number of animals in each group. ^#p < 0.01 compared with the control groups treated with vehicle, ^##p < 0.001 compared with the control groups treated with vehicle, *p < 0.01 compared with the MNU-treated group treated with vehicle, **p < 0.001 compared with the MNU-treated group treated with vehicle.