Figure 4: The effect of knockdown of DNMT1 on CYP gene expression in HepG2 cells.
From: Zebularine upregulates expression of CYP genes through inhibition of DNMT1 and PKR in HepG2 cells
(A) HepG2 cells were transfected with siRNA against DNMT1 or negative control. After 72 h transfection, the cells were harvested and western blot analysis was performed to measure the DNMT1 protein level. GAPDH was used as a loading control. (B) Expression levels of CYP1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 and 3A4 in HepG2 cells transfected with siRNA against DNMT1 were examined by qRT-PCR. The comparative threshold cycle (Ct) method was used to determine the relative ratio of expression for each gene, which was corrected against HPRT1. Each data point represents the mean ± SEM of the values obtained from three independent experiments. The expression level of HepG2 transfected with negative control was considered to be 1.0 expression. *p < 0.05, compared to control HepG2 cells.
