Figure 5: The effect of PKR inhibitor on CYP gene expression in HepG2 cells.
From: Zebularine upregulates expression of CYP genes through inhibition of DNMT1 and PKR in HepG2 cells
(A–I) HepG2 cells were exposed to PKR inhibitor for 72 h. Expression levels of CYP1A1 (A), 1A2 (B), 2A6 (C), 2B6 (D), 2C9 (E), 2C19 (F), 2D6 (G), 2E1 (H) and 3A4 (I) were examined by qRT-PCR. The comparative threshold cycle (Ct) method was used to determine the relative ratio of expression for each gene, which was corrected with HPRT1. Each data point represents the mean ± SEM of the values obtained from three independent experiments. The expression level measured after exposure to 0 μM PKR inhibitor was considered to be 1.0 expression. *p < 0.05, compared to 0 μM.
