Figure 8: The effect of zebularine treatment on evaluation of drug toxicity.
From: Zebularine upregulates expression of CYP genes through inhibition of DNMT1 and PKR in HepG2 cells
(A,B) Comparison of cell viability with and without zebularine. After pretreatment with zebularine for 72 h, cells were exposed to acetaminophen (A) or aflatoxin B1 (B) for the next 72 h and then examined for cell viability. Each data point represents the mean ± SEM of the values obtained from three independent experiments. Zebularine-treated and untreated cell cultures exposed to 0 mM acetaminophen or 0 μM aflatoxin B1 were considered to be 100% viable. *p < 0.05, compared to the untreated (-zebularine) group. (C) Analysis of CYP2E1 and CYP3A4 protein levels after exposure to zebularine. After 72 h zebularine exposure, the cells were harvested and western blot analysis was performed to measure the CYP2E1 or 3A4 protein level. GAPDH was used as a loading control.
